A modern, simple, and economical accessory for continuous L-format measurement of steady-state fluorescence anisotropy

In this study, the pros and cons of the most relevant L-format devices reported in the literature for measuring steady-state fluorescence polarization/anisotropy are identified. Combining all this information, and with the use of modern elements for the acquisition, treatment, and recording of signals, a modern, simple, and economical L format accessory is implemented to rapidly and continuously record steady-state fluorescence anisotropy. This device can be adapted to the majority of the commercial spectrofluorometers (or fluorometers). During the measurement, the emission polarizer is in permanent rotation by means of a Gimbal brushless DC motor, and as a result the recorded fluorescence signal is sinusoidal. The maximums and minimums of this signal, which are obtained with the help of LabVIEW tools, allow recording the fluorescence anisotropy. The LabVIEW applications developed for this investigation are freely available, so it is not necessary to have LabVIEW software.

Automated summary

This study identifies the pros and cons of relevant L-format devices for measuring steady-state fluorescence polarization/anisotropy and develops a modern, simple, and economical L-format accessory for recording fluorescence anisotropy. The device is compatible with most commercial fluorometers and LabVIEW tools are utilized for signal recording.