Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 414, Issue 5, Pages 1933-1947Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03829-z
Keywords
LC-MS/MS; Sum of MRM (SMRM); Bioanalysis; Large molecules; Charge state distribution
Funding
- National Institute of Allergy and Infectious Diseases, National Institutes of Health [HHSN272201100022I]
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LC-MS/MS provides a simple and efficient means for the measurement of analytes in biological matrices, but can pose a challenge for large molecules like proteins and peptides which need to be in an ionized form for detection. The MRM method minimizes interference from endogenous molecules with the same molecular weight of the precursor ion, but sacrifices signal intensity compared to precursor ion intensity.
Liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) provides a simple and efficient means for the measurement of analytes in biological matrices with high selectivity and specificity. LC-MS/MS plays an important role in the pharmaceutical industry and biomedical research, but it requires analytes to be in an ionized form in order to be detected. This can pose a challenge for large molecules such as proteins and peptides, because they can exist in multiple charged forms, and this will reduce the total analyte signal by distributing it into multiple ion peaks with a different number of charges in a mass spectrum. In conventional LC-MS/MS analysis of such macromolecules, one charged form is selected as the precursor ion which is then fragmented by collision-induced dissociation (CID) in MS/MS to generate product ions, a process referred to as multiple-reaction monitoring (MRM). The MRM method minimizes interference from endogenous molecules within biological matrices that share the same molecular weight of the precursor ion, but at the expense of signal intensity as compared to precursor ion intensity. We describe here an approach to boost detection sensitivity and expand dynamic range in the quantitation of large molecules while maintaining analytical specificity using summation of MRM (SMRM) transitions and LC separation technique.
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