Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 414, Issue 4, Pages 1677-1689Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03794-7
Keywords
Carbosilane dendron magnetic nanoparticles; Protein; Sustainability; Purification; Extraction
Funding
- CRUE-CSIC
- Springer Nature
- Spanish Ministry of Science and Innovation [PID2020-114891RBI00]
- Comunidad of Madrid
- FSE program [S2018/BAA-4393]
- FEDER program [S2018/BAA-4393]
- MINECO [CTQ2017-86224-P]
- VI National RAMP
- DAMP
- I Plan 2008-2011
- Iniciativa Ingenio 2010
- Consolider Program
- CIBER Actions
- Instituto de Salud Carlos III
- European Regional Development Fund
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This study proposes a more sustainable alternative for protein extraction using magnetic nanoparticles coated with carboxylate-terminated carbosilane dendrons. It investigates the interaction of proteins with MNPs under different conditions and the suitable disruption of interactions post-extraction. Additionally, the method was successfully applied to the recovery/purification of proteins from food byproducts.
Extraction/purification of proteins, at both analytical and industrial levels, is a limiting step that usually requires the use of organic solvents and involves tedious work and a high cost. This work proposes a more sustainable alternative based on the use of magnetic nanoparticles (MNPs) coated with carboxylate-terminated carbosilane dendrons. MNPs coated with first- and second-generation carbosilane dendrons and bare MNPs were employed for the extraction of proteins with different molecular weights and charges. Interaction of proteins with MNPs significantly varied with the pH, the protein, and the dendron generation (different sizes and number of charges in the periphery). Optimal dendron:protein molar ratios and suitable conditions for disrupting interactions after protein extraction were also researched. Second-generation dendron-coated MNPs showed 100% retention capability for all proteins when using acidic conditions. They were reused without losing magnetism or interaction capacity after a disruption of protein-dendron interactions with 0.2% SDS at 100 degrees C for 10 min. The capacity of dendron-coated MNPs was successfully applied to the recovery/purification of proteins from two food byproducts, olive seeds and cheese whey.
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