Journal
ANALYTICA CHIMICA ACTA
Volume 1184, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2021.339011
Keywords
Multicellular tumor spheroids; MALDI-MSI; Lipidomics; Hydroxychloroquine; Lipid metabolites
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Funding
- Kwok Chung Bo Fun Charitable Fund
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In this study, MALDI-MSI was used to analyze A549 lung cancer multicellular spheroids, revealing that HCQ treatment altered the spatial composition and distribution of lipids in the inner and outer regions of the spheroids.
Three-dimensional (3D) multicellular tumor spheroids (MCTS) that mimic the complex tumor micro environment provide a good platform for in vitro study of drug and endogenous metabolites. Hydroxychloroquine (HCQ) has shown anti-tumor activity in a variety of tumor models. However, the effect of the drug on the alteration of lipid metabolism spatial composition and distribution in the MCTS model is not clear. Herein, we utilized matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) in the analysis of A549 lung cancer multicellular spheroids to investigate the in situ spatial distribution of HCQ and its effect on lipid metabolism. We have successfully observed the spatial variations of HCQ in the inner region of the spheroid at different drug-treated time points. The MSI results also demonstrated that HCQ treatment altered the spatial composition of lipids in the inner and outer regions of treated spheroids. Furthermore, the lipidomic results showed that the identified phosphatidylcholines (PC), lysophosphatidylcholines (LPC), phosphatidylethanolamines (PE), lysophosphatidylethanolamines (LPE), phosphatidylinositols (PI), ceramides (Cer), glucosylceramides (CerG), and diglycerides (DG) were significantly up-regulated, and phosphatidylglycerol (PG) and triglycerides (TG) were remarkable down-regulated. MSI method combined with LC-MS/MS profiling of endogenous metabolites can obtain more detailed information about how spheroids respond to drug and spatial distribution information, thus fostering a better understanding of the relationship between drug-altered lipid metabolism and cancer microenvironment. (c) 2021 Elsevier B.V. All rights reserved.
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