Journal
ANALYTICA CHIMICA ACTA
Volume 1188, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2021.339167
Keywords
ATP-sensitive potassium channel; Smart copolymer; Nanochannels
Categories
Funding
- National Natural Science Foundation of China [21974048, 21635003, 92056203]
- Science and Technology Commission of Shanghai Municipality [19ZR1414600]
- Fundamental Research Funds for the Cen-tral Universities
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A smart copolymer modified nanochannel was designed to mimic the structure and mechanism of K-ATP channels, achieving ATP responsiveness and demonstrating a possible mechanism for ATP gating.
ATP-sensitive potassium (KATP) channels couple intracellular metabolism to the electrical activity by regulating K+ flux across the plasma membrane, thus playing an important role in both normal and pathophysiology. To understand the mechanism of ATP regulating biological ion channels, developing an ATP-responsive artificial nanochannel is an appealing but challenging topic because K-ATP channel is a heteromultimer of two subunits (potassium channel subunit (Kir6.x) and sulfonylurea receptor (SUR)) and exhibit dynamic functions with adjustability and reversibility. Inspired by the structure of K-ATP channels, we designed a smart copolymer modified nanochannel that may address the challenge. In the tricomponent poly(N-isopropylacrylamide) (PNIPAAm, PNI)-based copolymer system, phenylthiourea was used to bind the phosphate units of nucleotides and phenylboronic acid was introduced to combine the pentose ring of the nucleoside unit. Besides, a-COOH group with electron-withdrawing property was added into the phenylthiourea units, which may promote the hydrogen-bond-donating ability of thiourea. Specially, the smart copolymer not only provided static binding sites for recognition but also translated the recognition of ATP into their dynamic conformational transitions by changing the hydrogen-bonding environments surrounding PNIPAAm chains, thus achieving the gating function of nanochannel, which resembled the integration and coordination of Kir6.x and SUR units in active K-ATP. The ATP-regulated ion channel exhibited excellent stability and reversibility. This study is the first example showing how to learn from nature to assemble the ATP-responsive artificial nanochannel and demonstrate the possible mechanism of ATP gating. (C) 2021 Elsevier B.V. All rights reserved.
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