Journal
ANALYTICA CHIMICA ACTA
Volume 1183, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2021.338980
Keywords
Fluorescent probe; Dual-emission; Hypochlorous acid; Nitric oxide; Cell imaging
Categories
Funding
- National Natural Sci-ence Foundation of China [NSFC 21768004]
- National Students'Platform for Innovation and Entrepreneurship Training Program of China
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A new fluorescent probe QBN was developed for detection of HOCl and NO, and successfully achieved simultaneous imaging of both exogenous and endogenous HOCl and NO in live cells.
Both reactive oxygen species (ROS) and reactive nitrogen species (RNS) are inevitably produced during normal human metabolism. Various ROS and RNS together form tangled networks that play important roles in many physiological and pathological processes. Here we used 1,8-naphthalene diamine as a reactive group to develop a fluorescent probe, N-[2-(6-phenylethynyl)quinolinylmethyl]-1,8-diamino naphthalene (QBN), for HOCl and NO. QBN showed a turn-on fluorescent response at 464 nm to HOCl in the range of 0-75 mu M with rapid responding time (10 s) and detection limit (0.11 +/- 0.03 mM). Furthermore, a turn-on fluorescent responses at 512 nm to NO in the range of 0-40 mu M with responding time (20 s) and detection limit (25.7 +/- 3.4 nM) was found. The response mechanisms of QBN to HOCl and NO were discussed based on mass analysis of the different products. The dual-channel probe was then successfully applied for simultaneous imaging of both exogenous and endogenous HOCl and NO in live cells. (C) 2021 Elsevier B.V. All rights reserved.
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