Journal
ANALYTICA CHIMICA ACTA
Volume 1183, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2021.338956
Keywords
On-surface adherent cell assay; PPAR gamma and Cytotox CALUX (R); Saussurea costus; Ginseng; Luciferase expressing reporter gene
Categories
Funding
- German Research Foundation [INST 162/471-1 FUGG, INST 162/536-1 FUGG]
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This study demonstrates the novel combination of chromatographic separations with human cancer cells as biological detectors, leading to the discovery of cytotoxic substances in herbal samples and the detection of specific compounds targeting receptor-mediated signaling pathways. High-resolution mass spectrometry and fragmentation were used to further characterize the discovered compounds.
For the first time, a human cancer cell line was shown to grow and be functionally active on the particulate porous adsorbent surface of separated sample mixtures. This allowed the novel combination of chromatographic separations with human cells as biological detector. As exemplary screening for cancer treatment drugs, cytotoxic substances were directly discovered in Saussurea costus and ginseng samples using the Cytotox CALUX (R) osteosarcoma cells (with luciferase expressing reporter gene) as detector. In addition, rosiglitazone and pioglitazone were detected as luminescent zones upon binding to the PPAR gamma receptor expressed in the respective CALUX cell line that was grown on the surface of the adsorbent. This demonstrates the ability to address receptor-mediated signaling with this method, and opens the perspective to use our novel bioimaging method to identify bioactive molecules targeting a wide range of pathways with toxicological, pharmaceutical and nutraceutical relevance. The new bioimaging directly pointed to individual effective compounds in multi-component mixtures. Furthermore, discovered effective compounds were directly characterized by online elution to high-resolution mass spectrometry and fragmentation. (C) 2021 Elsevier B.V. All rights reserved.
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