Molecular detection of avian haemosporidian parasites in biting midges (Diptera: Ceratopogonidae) from Thailand

Culicoides biting midges are vectors of many haemosporidian parasite species. These parasites are found in several wild and domestic avian species in Thailand but knowledge of the insect vectors is very limited. In this study, a molecular approach was used to detect haemosporidian parasites in six biting midge species in Thailand. A total of fifteen cytochrome b haplotypes of three haemosporidian parasites were detected from 1,165 specimens of six different Culicoides species. Comparisons of these sequences with those recorded in a public database revealed that they were comprised sequences of three species, Leucocytozoon sp., Plasmodium juxtanucleare and P. gallinaceum. All of these haemosporidian parasite species were detected in Culicoides mahasarakhamense Pramual, Jomkumsing, Piraonapicha, & Jumpato while P. juxtanucleare was also detected in C. huffi Causey and C. guttifer Meijere and Leucocytozoon sp. were also detected in C. guttifer. All of these parasites are commonly found in chickens in agreement with information that these biting midge species will bite chickens. Detection DNA of Leucocytozoon sp. in biting midges reported here provides the second record of this parasitic genus infecting Culicoides. This study also provides the first records of P. juxtanucleare and P. gallinaceum in biting midge species. Further investigation is required to determine whether Culicoides biting midge species are competent vectors of these parasites.

Automated summary

This study used a molecular approach to detect haemosporidian parasites in six biting midge species in Thailand, finding three species of these parasites. The study suggests that biting midges may be competent vectors of these parasites, with further investigation needed for confirmation.