4.6 Article

Structural Insight into the Reaction Mechanism of Ketosynthase-Like Decarboxylase in a Loading Module of Modular Polyketide Synthases

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

Structures of LnmK, a Bifunctional Acyltransferase/Decarboxylase, with Substrate Analogues Reveal the Basis for Selectivity and Stereospecificity

Lee M. Stunkard et al.

Summary: The study reveals that LnmK enzyme uses substrate-assisted catalysis to carry out decarboxylation and acyl transfer reactions, providing support for the biosynthesis of polyketides. The molecular structures of substrate analogues bound to LnmK help in understanding substrate specificity, stereospecificity, and catalytic mechanism, showcasing the potential of these analogues in studying enzymes with complex structure-function relationships.

BIOCHEMISTRY (2021)

Article Chemistry, Physical

Structure and Mechanistic Analyses of the Gating Mechanism of Elongating Ketosynthases

Jeffrey T. Mindrebo et al.

Summary: The study focuses on the mechanism of ketosynthases, demonstrating the importance of interactions between active site loops and acyl carrier proteins in catalysis. It also provides evidence for the role of gating in the overall KS condensation reaction and explores the catalytic importance of these loops through mutagenesis and investigation of chimeric FabF constructs. These findings contribute to a better understanding of the KS mechanism and can inform future engineering efforts in fatty acid synthases and related polyketide synthases.

ACS CATALYSIS (2021)

Article Biochemistry & Molecular Biology

Type I fatty acid synthase trapped in the octanoyl-bound state

Alexander Rittner et al.

PROTEIN SCIENCE (2020)

Article Biochemistry & Molecular Biology

Structural basis for selectivity in a highly reducing type II polyketide synthase

Danyao Du et al.

NATURE CHEMICAL BIOLOGY (2020)

Article Chemistry, Multidisciplinary

Sulfonate/Nitro Bearing Methylmalonyl-Thioester Isosteres Applied to Methylmalonyl-CoA Decarboxylase Structure-Function Studies

Lee M. Stunkard et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2019)

Article Biochemistry & Molecular Biology

Molecular basis for interactions between an acyl carrier protein and a ketosynthase

Jacob C. Milligan et al.

NATURE CHEMICAL BIOLOGY (2019)

Review Chemistry, Multidisciplinary

The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines

Adrian T. Keatinge-Clay

CHEMICAL REVIEWS (2017)

Article Biochemistry & Molecular Biology

Roles of Conserved Active Site Residues in the Ketosynthase Domain of an Assembly Line Polyketide Synthase

Thomas Robbins et al.

BIOCHEMISTRY (2016)

Article Biochemistry & Molecular Biology

A Close Look at a Ketosynthase from a Trans-Acyltransferase Modular Polyketide Synthase

Darren C. Gay et al.

STRUCTURE (2014)

Article Biochemistry & Molecular Biology

Cyanobacterial Polyketide Synthase Docking Domains: A Tool for Engineering Natural Product Biosynthesis

Jonathan R. Whicher et al.

CHEMISTRY & BIOLOGY (2013)

Article Multidisciplinary Sciences

Engineering the acyltransferase substrate specificity of assembly line polyketide synthases

Briana J. Dunn et al.

JOURNAL OF THE ROYAL SOCIETY INTERFACE (2013)

Article Biotechnology & Applied Microbiology

Structure-Guided Expansion of the Substrate Range of Methylmalonyl Coenzyme A Synthetase (MatB) of Rhodopseudomonas palustris

Heidi A. Crosby et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2012)

Review Biochemistry & Molecular Biology

The structures of type I polyketide synthases

Adrian T. Keatinge-Clay

NATURAL PRODUCT REPORTS (2012)

Article Biochemical Research Methods

iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM

T. Geoff G. Battye et al.

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (2011)

Article Biochemical Research Methods

Molecular replacement with MOLREP

Alexei Vagin et al.

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (2010)

Article Biochemical Research Methods

MolProbity: all-atom structure validation for macromolecular crystallography

Vincent B. Chen et al.

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2010)

Article Biochemical Research Methods

XDS

Wolfgang Kabsch

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (2010)

Article Chemistry, Multidisciplinary

Structure and mechanism of an unusual malonate decarboxylase and related racemases

Krzysztof Okrasa et al.

CHEMISTRY-A EUROPEAN JOURNAL (2008)

Article Multidisciplinary Sciences

The crystal structure of a mammalian fatty acid synthase

Timm Maier et al.

SCIENCE (2008)

Article Multidisciplinary Sciences

GNAT-like strategy for polyketide chain initiation

Liangcai Gu et al.

SCIENCE (2007)

Article Multidisciplinary Sciences

The 2.7-Å crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase

Yinyan Tang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Article Multidisciplinary Sciences

Platensimycin is a selective FabF inhibitor with potent antibiotic properties

J Wang et al.

NATURE (2006)

Article Biochemical Research Methods

Coot:: model-building tools for molecular graphics

P Emsley et al.

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2004)

Article Biochemistry & Molecular Biology

Reconstituting modular activity from separated domains of 6-deoxyerythronolide B synthase

CY Kim et al.

BIOCHEMISTRY (2004)

Article Biochemistry & Molecular Biology

Mechanism of the β-ketoacyl synthase reaction catalyzed by the animal fatty acid synthase

A Witkowski et al.

BIOCHEMISTRY (2002)

Article Biochemistry & Molecular Biology

Substrate specificity of the loading didomain of the erythromycin polyketide synthase

J Lau et al.

BIOCHEMISTRY (2000)