Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 14, Issue 3, Pages 4714-4724Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c21173
Keywords
long-chain nucleic acid detection; magnetic modulation; surface-enhanced Raman signal; DNA flexibility; plasmon coupling distance
Funding
- Shenzhen-Hong Kong-Macao Science and Technology Plan Project (Category C) [SGDX2020110309260000]
- Research Grants Council (RGC) of Hong Kong Collaborative Research Grant [C5110-20GF]
- Department of Biomedical Engineering [0033912]
- Start-up Fund for RAPs under the Strategic Hiring Scheme, the Hong Kong Polytechnic University (PolyU, University Grant Council) [0035876]
- National Natural Science Foundation of China (NSFC) [31771077]
- Research Grants Council (RGC) of Hong Kong General Research Grant [PolyU 15214619E, PolyU 15210818E]
- Hong Kong Polytechnic University Internal Fund [1-ZE1E]
- University Research Facility in Life Sciences of PolyU
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This study reports a magnetic-responsive substrate for ultrasensitive and highly selective detection of the N gene of SARS-CoV-2. The platform can reversibly shorten the coupling distance and enhance SERS signals, leading to a 10-fold increase in the detection limit.
Surface-enhanced Raman scattering (SERS)-based biosensors are promising tools for virus nucleic acid detection. However, it remains challenging for SERS-based biosensors using a sandwiching strategy to detect long-chain nucleic acids such as nucleocapsid (N) gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) because the extension of the coupling distance (CD) between the two tethered metallic nanostructures weakens electric field and SERS signals. Herein, we report a magnetic-responsive substrate consisting of heteoronanostructures that controls the CD for ultrasensitive and highly selective detection of the N gene of SARS-CoV-2. Significantly, our findings show that this platform reversibly shortens the CD and enhances SERS signals with a 10-fold increase in the detection limit from 1 fM to 100 aM, compared to those without magnetic modulation. The optical simulation that emulates the CD shortening process confirms the CD-dependent electric field strength and further supports the experimental results. Our study provides new insights into designing a stimuli-responsive SERS-based platform with tunable hot spots for long-chain nucleic acid detection.
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