Egyptian Olea europaea leaves bioactive extract: Antibacterial and wound healing activity in normal and diabetic rats

Background and aim: In vitro activity evaluation of Egyptian Olea europaea leaves extracts, and in vivo healing activity assessment of the newly prepared ointment of Olea europaea leaves extracts mingled with Shea butter. Experimental procedure: Different extraction methods and solvents were used to extract Egyptian Olea europaea bioactive agent(s). Antibacterial, scavenging activity and in-vivo evaluation of wound repair potentiality of Olea europaea extract were examined in normal and diabetic experimental rat models with induced circular excisions. Results and conclusion: Olive leaves extract of Tanta was selected as the most active agent against Methicillin-resistant S. aureus (MRSA), with MIC value 15.6 mu g/ml. Moreover, checkerboard dilution technique approved that the interaction between Tanta LEM crude extract and Ciprofloxacin was synergistic. Scavenging activity of the extract against DPPH free radicals was 87.55% at concentration of 50 mu g/ml. In vivo normal and diabetic experimental rats treated with Shea butter: Tanta LEM extract (1:3 w/v) showed the maximum wound contraction and healing activity. (C) 2021 Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC.

Automated summary

The study evaluated the activity of Egyptian Olea europaea leaves extracts and assessed the healing activity of the ointment prepared with Shea butter. Results showed that the Tanta Olive leaves extract exhibited the highest activity against MRSA and showed synergy with Ciprofloxacin, with high scavenging activity against free radicals. In vivo experiments on rats demonstrated that the combination of Shea butter and Tanta LEM extract had the best wound contraction and healing activity.