4.0 Article

Hb San Cataldo [144(HC1)LysThr; HBB: C.434A>C]: A New Hemoglobin Variant with Increased Affinity for Oxygen

Journal

HEMOGLOBIN
Volume 40, Issue 4, Pages 223-227

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/03630269.2016.1182550

Keywords

Genetic counseling; hemoglobinopathies; hemoglobin (Hb) variant; increased oxygen affinity; screening program

Funding

  1. Piera and Franco Cutino Foundation, Palermo, Italy

Ask authors/readers for more resources

A 59-year-old Italian woman came to our center for revaluation of a previous diagnosis of polycythemia vera. The patient presented with a lifelong history of polycythemia, no increase in white blood cells (WBCs) and platelets, and a negative bone marrow biopsy. Analysis of hemoglobin (Hb) fractions showed an abnormal fast moving Hb component. We aimed to determine if this variant was the cause of polycythemia in this patient. A complete blood count (CBC) was performed by an automated cell counter and Hb fractions were determined by high performance liquid chromatography (HPLC). Standard stability tests and oxygen affinity evaluation were also performed. Genomic DNA was extracted from peripheral blood leukocytes using the phenol chloroform method and the entire -globin gene was analyzed by direct sequencing. At the hematological level, no anemia or hemolysis was observed but an abnormal Hb fraction was detected using cation exchange HPLC. Molecular analysis of the -globin gene showed heterozygosity for an AAG>ACG substitution at codon 144, resulting in a LysThr amino acid replacement. We demonstrated that this is a new Hb variant with increased oxygen affinity. Its altered physiology is caused by the reduction of 2,3-diphosphoglycerate (2,3-DPG) effects, due to an amino acid substitution in the central pocket near the C-terminal of the chain. We called this new variant Hb San Cataldo for the native city of proband.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.0
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available