Anti-inflammatory Activity of Two New Indole Alkaloids from the Stems of Naucleaofficinalis

Two new indole alkaloid derivatives (1, 2), together with six known indole alkaloids (3-8) were isolated from the 70% EtOH/H2O extract of the stem of Naucleaofficinalis. Their structures were determined on the basis of extensive analyses of spectroscopic data (IR, MS, 1D- and 2D-NMR). All the isolates were evaluated for their anti-inflammatory activities on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7, and all the compounds showed significant inhibitory activities with the IC50 values of 0.82, 6.45, 9.75, 1.34, 3.40, 2.69, 1.58, and 1.96m, compared to the positive drug control group aminoguanidine with an IC50 value of 1.80m, especially compound 1 had the most significant activity.