Journal
PHYSIOLOGICAL REPORTS
Volume 9, Issue 17, Pages -Publisher
WILEY
DOI: 10.14814/phy2.15004
Keywords
contractile function; inflammation; ischemia; myocardial biology; transplantation
Categories
Funding
- Thoracic Surgery Foundation-Southern Thoracic Surgical Association Research Award
- NIH [U01-AI132895, R01OD023138]
- Department of Defense [DoD W81XWH-19-RTTP-IDA]
Ask authors/readers for more resources
Cold ischemic preservation results in greater dysfunction, cell death, and inflammation in the left ventricle compared to the right ventricle of mouse hearts, with differential expression of inflammatory cytokines and inflammasome components. However, elevated IL-6 protein levels and transcriptional profiles are similar in both ventricles. Similar findings are observed in human donor hearts.
Background: Dysfunction and inflammation of hearts subjected to cold ischemic preservation may differ between left and right ventricles, suggesting distinct strategies for amelioration. Methods and Results: Explanted murine hearts subjected to cold ischemia for 0, 4, or 8 h in preservation solution were assessed for function during 60 min of warm perfusion and then analyzed for cell death and inflammation by immunohistochemistry and western blotting and total RNA sequencing. Increased cold ischemic times led to greater left ventricle (LV) dysfunction compared to right ventricle (RV). The LV experienced greater cell death assessed by TUNEL+ cells and cleaved caspase-3 expression (n = 4). While IL-6 protein levels were upregulated in both LV and RV, IL-1 beta, TNF alpha, IL-10, and MyD88 were disproportionately increased in the LV. Inflammasome components (NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3), adaptor molecule apoptosis-associated speck-like protein containing a CARD (ASC), cleaved caspase-1) and products (cleaved IL-1 beta and gasdermin D) were also more upregulated in the LV. Pathway analysis of RNA sequencing showed increased signaling related to tumor necrosis factor, interferon, and innate immunity with ex-vivo ischemia, but no significant differences were found between the LV and RV. Human donor hearts showed comparable inflammatory responses to cold ischemia with greater LV increases of TNF alpha, IL-10, and inflammasomes (n = 3). Conclusions: Mouse hearts subjected to cold ischemia showed time-dependent contractile dysfunction and increased cell death, inflammatory cytokine expression and inflammasome expression that are greater in the LV than RV. However, IL-6 protein elevations and altered transcriptional profiles were similar in both ventricles. Similar changes are observed in human hearts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available