The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles

Magnetic Fe3O4 nanoparticles (MNPs) have great potential in the nucleic acid delivery approach for therapeutic applications. Herein, the formation of a stable complex of iron oxide nanoparticles with oligonucleotides was investigated. Several factors, such as pH, buffer components, and oligonucleotides sequences, were chosen for binding efficiency studies and oligonucleotide binding constant calculation. Standard characterization techniques, such as dynamic light scattering, zeta potential, and transmission electron microscopy, provide MNPs coating and stability. The toxicity experiments were performed using lung adenocarcinoma A549 cell line and high reactive oxygen species formation with methylene blue assay. Fe3O4 MNPs complexes with oligonucleotides show high stability and excellent biocompatibility.

Automated summary

The study investigated the formation of a stable complex of iron oxide nanoparticles with oligonucleotides through different factors selection to enhance binding efficiency, and identified the complexes using standard characterization techniques. The experimental results showed that the complexes formed by Fe3O4 MNPs with oligonucleotides exhibited high stability and biocompatibility.