Journal
JOURNAL OF INTEGRATIVE MEDICINE-JIM
Volume 19, Issue 5, Pages 408-417Publisher
ELSEVIER
DOI: 10.1016/j.joim.2021.05.001
Keywords
Bufalin; Liver cancer; Epidermal growth factor receptor; Immunomicelles
Categories
Funding
- National Natural Science Foundation of China [81673739]
- National Key Research and Development Program of China [2018YFC1705305]
- Shanghai Development Office of Traditional Chinese Medicine [ZY[2018-2020]-FWTX-4010]
- Shanghai ''13th Five-year Plan Clinical Key Department-Department of Dermatology of Traditional Chinese Medicine [shslczdzk05001]
- Clinical Research Plan of SHDC [SHDC2020CR3097B]
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In this study, a dual targeting immunomicelles (DTIs) loaded with bufalin were designed and synthesized, which have features of temperature sensitivity, high stability, and slow drug release. Compared with free bufalin, the cellular uptake and cytotoxicity of DTIs-BF against liver cancer cells were significantly enhanced, showing better therapeutic effects.
Objective: Bufalin is an effective drug for the treatment of liver cancer. But its high toxicity, poor water solubility, fast metabolism and short elimination half-life limit its use in tumor treatment. How to make the drug accumulate in the tumor and reduce side effects while maintaining its efficacy are urgent problems to be solved. The goal of this study is to solve these problems. Methods: A copolymer with tunable poly-N-isopropylacrylamide and polylactic acid was designed and synthesized. The corresponding dual targeting immunomicelles (DTIs) loaded with bufalin (DTIs-BF) were synthesized by copolymer self-assembly in an aqueous solution. The size and structure of DTIs- BF were determined by ZetaSizer Nano-ZS and transmission electron microscopy. Then, its temperature sensitivity, serum stability, critical micelle concentration (CMC), entrapment efficiency (EE), drug release and non-cytotoxicity of blank block copolymer micelles (BCMs) were evaluated. Next, the effects of DTIs-BF on cellular uptake, cytotoxicity, and tumor cell inhibition were evaluated. Finally, the accumulation of DTIs-fluorescein isothiocyanate (FITC) and the in vivo anti-tumor effect were observed using an interactive video information system. Results: DTIs-BF had a small size, spherical shape, good temperature sensitivity, high serum stability, low CMC, high EE, and slow drug release. The blank BCMs had very low cytotoxicity. Compared with free bufalin, the in vitro cellular internalization and cytotoxicity of DTIs-BF against SMMC-7721 cells were significantly enhanced, and the effects were obviously better at 40 degrees C than 37 degrees C. In addition, the therapeutic effect on SMMC-7721 cells was further enhanced by the programmed cell death specifically caused by bufalin. When DTIs-FITC were injected intravenously in BALB/c nude mice bearing liver cancer, the accumulation of FITC was significantly increased in tumors. Conclusion: DTIs-BF is a potentially effective nano-formulation and has broad prospects in the clinical treatment of liver cancer. (C) 2021 Shanghai Yueyang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine. Published by ELSEVIER B.V. All rights reserved.
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