Journal
CHEMPHOTOCHEM
Volume 5, Issue 10, Pages 957-965Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cptc.202100109
Keywords
open source; photobiocatalysis; photocatalysis; photoenzymes; photoreactors; reaction engineering
Categories
Funding
- European Union [862081, 764920]
- Austrian Science Fund (FWF) [DOC 46-B21]
- University of Graz
- Field of Excellence BioHealth
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Photobiocatalysis is an emerging synthesis approach that is gaining attention, with only a few commercially available reactor systems due to its youth. A newly developed photoreactor allows parallel parameter screening for optimization, demonstrated in applications such as light-dependent decarboxylation and photosynthetic oxygen production.
Photobiocatalysis is an alternative approach in synthesis that has received much attention in the recent years. Due to the youth of the topic, only few reactor systems are commercially available. To allow a parallel parameter-screening approach as often used in the optimization of biocatalytic processes, a photoreactor was developed that can illuminate up to 24 samples at well-defined reaction conditions. The device's optical features and temperature regulation have been thoroughly characterized and its application was demonstrated in four examples, specifically three photobiocatalytic and one photocatalytic process: (i) Light-dependent decarboxylation using a photodecarboxylase; (ii) Reduction of protochlorophyllide using a protochlorophyllide oxidoreductase; (iii) Photosynthetic oxygen production performed by cyanobacteria; and (iv) (-)-Riboflavin-catalyzed (E/Z)-isomerization of cinnamic acid derivatives.
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