Journal
LIFE-BASEL
Volume 11, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/life11080812
Keywords
abiotic stress; photosystem II; extreme high light; NPQ; LED
Categories
Funding
- Brazilian National Counsel of Technological and Scientific Development (CNPq) [249096/2013-7]
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN 3355743-13]
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The study reveals different responses of plants to extreme high-light stress under blue light and red light. Blue light stimulates a more efficient energy dissipation mechanism, while red light increases the abundance of photosystem II and oxygen-evolving complex proteins. The impact of extreme high-light stress on the proteomic level is wavelength-dependent, with different wavelengths leading to varied responses in plants.
Plants pigments, such as chlorophyll and carotenoids, absorb light within specific wavelength ranges, impacting their response to environmental light changes. Although the color-specific response of plants to natural levels of light is well described, extreme high-light stress is still being discussed as a general response, without considering the impact of wavelengths in particular response processes. In this study, we explored how the plant proteome coordinated the response and recovery to extreme light conditions (21,000 mu mol m(-2) s(-1)) under different wavelengths. Changes at the protein and mRNA levels were measured, together with the photosynthetic parameters of plants under extreme high-light conditions. The changes in abundance of four proteins involved in photoinhibition, and in the biosynthesis/assembly of PSII (PsbS, PsbH, PsbR, and Psb28) in both light treatments were measured. The blue-light treatment presented a three-fold higher non-photochemical quenching and did not change the level of the oxygen-evolving complex (OEC) or the photosystem II (PSII) complex components when compared to the control, but significantly increased psbS transcripts. The red-light treatment caused a higher abundance of PSII and OEC proteins but kept the level of psbS transcripts the same as the control. Interestingly, the blue light stimulated a more efficient energy dissipation mechanism when compared to the red light. In addition, extreme high-light stress mechanisms activated by blue light involve the role of OEC through increasing PsbS transcript levels. In the proteomics spatial analysis, we report disparate activation of multiple stress pathways under three differently damaged zones as the enriched function of light stress only found in the medium-damaged zone of the red LED treatment. The results indicate that the impact of extreme high-light stress on the proteomic level is wavelength-dependent.
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