Journal
CHEMOSENSORS
Volume 9, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/chemosensors9070165
Keywords
BODIPY; ferric ions; fluorescence; bioimaging; pyridyl group
Funding
- National Research Council of Thailand (NRCT) [NRCT5-TRG630014-02]
- National Nanotechnology center [P2051931]
- Research Network of NANOTEC (RNN) program of the National Nanotechnology Center (NANOTEC), NSTDA, Ministry of Higher Education, Science, Research, and Innovation, Thailand [P1851658]
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A novel pyridylhydrazone-tethered BODIPY was synthesized and characterized for the selective detection of Fe3+ through fluorescent enhancement. The complex formation between BODIPY-PH and Fe3+ was reversible and showed chelation-enhanced fluorescence effect. The BODIPY-PH displayed good permeability, low toxicity, and enhanced fluorescent signal in cells incubated with Fe3+, indicating its potential for Fe3+ analysis in cellular matrix.
A novel pyridylhydrazone-tethered BODIPY (BODIPY-PH) was synthesized, fully characterized via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopic (FTIR), and single-crystal X-ray diffraction (SC-XRD) techniques, and developed for the selective detection of Fe3+ through fluorescent enhancement process. This derivative showed 1:1 binding with Fe3+ in an acetonitrile-water mixture (1:9 v/v) with the binding constant (K) of 5.4 x 10(4) M-1 and the limit of detection of 0.58 mu M. The Fe3+ complexation reaction has been proved to be a reversible process and could be effectively repeated up to three cycles. The electronic properties of BODIPY-PH and its Fe3+ complex modeled by the density functional theory (DFT) method suggested the presence of chelation-enhanced fluorescence (CHEF) effect in the Fe3+ binding reaction. The X-ray absorption spectroscopy (XAS) probed at Fe K-edge confirmed the complex formation between BODIPY-PH and the Fe3+ in an octahedral geometry. Finally, bioimaging against human embryonic kidney (Hek293) cell, through confocal fluorescence microscopic technique indicated that the BODIPY-PH displayed good permeability and low toxicity toward the tested cell lines and showed enhanced fluorescent signal in the cells incubated with Fe3+ proving its capability for Fe3+ analysis in cellular matrix.
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