A multicolor and ratiometric fluorescent sensing platform for metal ions based on arene-metal-ion contact

Fluorescent probes can detect metal ions with high sensitivity, but their design typically relies on a limited number of sensing mechanisms. Here the use of arene-metal-ion contact as a sensing mechanism allows ratiometric detection of metal ions across a broad wavelength range. Despite continuous and active development of fluorescent metal-ion probes, their molecular design for ratiometric detection is restricted by the limited choice of available sensing mechanisms. Here we present a multicolor and ratiometric fluorescent sensing platform for metal ions based on the interaction between the metal ion and the aromatic ring of a fluorophore (arene-metal-ion, AM, coordination). Our molecular design provided the probes possessing a 1,9-bis(2 '-pyridyl)-2,5,8-triazanonane as a flexible metal ion binding unit attached to a tricyclic fluorophore. This architecture allows to sense various metal ions, such as Zn(II), Cu(II), Cd(II), Ag(I), and Hg(II) with emission red-shifts. We showed that this probe design is applicable to a series of tricyclic fluorophores, which allow ratiometric detection of the metal ions from the blue to the near-infrared wavelengths. X-ray crystallography and theoretical calculations indicate that the coordinated metal ion has van der Waals contact with the fluorophore, perturbing the dye's electronic structure and ring conformation to induce the emission red-shift. A set of the probes was useful for the differential sensing of eight metal ions in a one-pot single titration via principal component analysis. We also demonstrate that a xanthene fluorophore is applicable to the ratiometric imaging of metal ions under live-cell conditions.

Automated summary

A fluorescent sensing platform based on arene-metal-ion contact was developed for multicolor and ratiometric detection of metal ions across a broad wavelength range. The flexible molecular design allows for the detection of various metal ions with emission red-shifts. X-ray crystallography and theoretical calculations revealed the mechanism behind the emission red-shift induced by the coordinated metal ions. The probes showed promising differential sensing capabilities and applicability for ratiometric imaging of metal ions under live-cell conditions.