Journal
PHARMACEUTICALS
Volume 14, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/ph14060511
Keywords
MERS-CoV; egg yolk antibodies; antiviral; S-protein; in vivo; in vitro
Categories
Funding
- Research Program on Emerging and Reemerging Infectious Diseases at the Japan Agency for Medical Research and Development [JP19fk0108058]
- Ministry of Education, Culture, Sports, Science, and Technology in Japan [18H02665, 19K08945]
- Grants-in-Aid for Scientific Research [18H02665, 19K08945] Funding Source: KAKEN
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In this study, chicken egg yolk antibodies specific to the MERS-CoV spike protein were developed and shown to efficiently neutralize MERS-CoV infection both in vitro and in vivo.
Identified in 2012, the Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe and often fatal acute respiratory illness in humans. No approved prophylactic or therapeutic interventions are currently available. In this study, we developed chicken egg yolk antibodies (IgY Abs) specific to the MERS-CoV spike (S) protein and evaluated their neutralizing efficiency against MERS-CoV infection. S-specific IgY Abs were produced by injecting chickens with the purified recombinant S protein of MERS-CoV at a high titer (4.4 mg/mL per egg yolk) at week 7 post immunization. Western blotting and immune-dot blot assays demonstrated specific binding to the MERS-CoV S protein. In vitro neutralization of the generated IgY Abs against MERS-CoV was evaluated and showed a 50% neutralizing concentration of 51.42 mu g/mL. In vivo testing using a human-transgenic mouse model showed a reduction of viral antigen positive cells in treated mice, compared to the adjuvant-only controls. Moreover, the lung cells of the treated mice showed significantly reduced inflammation, compared to the controls. Our results show efficient neutralization of MERS-CoV infection both in vitro and in vivo using S-specific IgY Abs. Clinical trials are needed to evaluate the efficiency of the IgY Abs in camels and humans.
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