Journal
PHARMACEUTICALS
Volume 14, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/ph14080771
Keywords
Juglans mandshurica Maxim; anti-inflammatory activity; NF-kappa B; MAPK; zebrafish larvae
Categories
Funding
- Korea Basic Science Institute [C140360, K111000]
- National Research Council of Science & Technology (NST), Republic of Korea [C140360] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The study demonstrates that HHMP significantly inhibits LPS-induced inflammatory responses by suppressing NO and prostaglandin production, regulating protein expression, and inhibiting NF-kappa B and MAPK activation. This suggests HHMP's potential as a natural anti-inflammatory agent.
Juglans mandshurica Maxim., a traditional folk medicinal plant, is widely distributed in Korea and China. In our previous study, we isolated a new phenylpropanoid compound, 4-((1R,2R)-3-hydroxy-1-(4-hydroxyphenyl)-1-methoxypropan-2-yl)-2-methoxyphenol (HHMP), from J. mandshurica. In the present study, we evaluated the anti-inflammatory activity of HHMP on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and zebrafish larvae. HHMP significantly inhibited LPS-induced nitric oxide (NO) and prostaglandin E-2 production in a dose-dependent manner. Moreover, HHMP treatment considerably suppressed LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2. We also demonstrated the mechanisms of HHMP inhibition of inflammatory responses in LPS-stimulated RAW 264.7 cells via Western blot analysis and immunofluorescence staining. Furthermore, HHMP significantly inhibited NO production in LPS-stimulated zebrafish larvae. Consequently, we established that HHMP significantly inhibited the LPS-induced activation of NF-kappa B and MAPK and the nuclear translocation of p65 in RAW 264.7 cells. Taken together, our findings demonstrate the effect of HHMP on LPS-induced inflammatory responses in vitro and in vivo, suggesting its potential to be used as a natural anti-inflammatory agent.
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