Journal
ISCIENCE
Volume 24, Issue 9, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.isci.2021.103072
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Funding
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence [Strategy-EXC 2089/1-390776260]
- DFG [INST 86/1904-1 FUGG, TI 329/9-2]
- BMBF [13N14336, 03VP03891]
- Humboldt Research Fellowships from the Alexander von Humboldt Foundation
- European Union [843179]
- Associazione Italiana per la Ricerca sul Cancro, AIRC [21965]
- European Research Council, ERC [819160]
- Marie Curie Actions (MSCA) [843179] Funding Source: Marie Curie Actions (MSCA)
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DNA nanotechnology integrates DNA origami nanoantennas with label-free antibody detection, demonstrating single-antibody detection by incorporating a nanoswitch in the nanoantenna's plasmonic hotspot. The signal generated by the antibody is additionally amplified, enabling the detection of single antibodies on a portable smartphone microscope. This study expands the application of fluorescence-enhanced biosensing beyond nucleic acids.
DNA nanotechnology offers new biosensing approaches by templating different sensor and transducer components. Here, we combine DNA origami nanoantennas with label-free antibody detection by incorporating a nanoswitch in the plasmonic hotspot of the nanoantenna. The nanoswitch contains two antigens that are displaced by antibody binding, thereby eliciting a fluorescent signal. Single-antibody detection is demonstrated with a DNA origami integrated anti-digoxigenin antibody nanoswitch. In combination with the nanoantenna, the signal generated by the antibody is additionally amplified. This allows the detection of single antibodies on a portable smartphone microscope. Overall, fluorescence-enhanced antibody detection in DNA origami nanoantennas shows that fluorescence-enhanced biosensing can be expanded beyond the scope of the nucleic acids realm.
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