Use of Wheat Straw for Value-Added Product Xylanase by Penicillium chrysogenum Strain A3 DSM105774

The present work highlights the valorization of the bulky recalcitrant lignocellulose byproduct wheat straw (WS) for the enhanced production of value-added xylanase by the locally sourced novel Penicillium chrysogenum strain A3 DSM105774 for the first time. The optimized production of xylanase by submerged state of fermentation of WS was achieved using a three-step statistical and sequential approach: one factor at a time (OFAT), Plackett-Burman design (PBD), and Box Behnken design (BBD). Incubation temperature (30 degrees C), WS, and ammonium sulphate were the key determinants prompting xylanase production; inferred from OFAT. The WS concentration (%(w/v)), yeast extract concentration (%(w/v)), and initial pH of the production medium imposed significant effects (p <= 0.05) on the produced xylanase, realized from PBD. The predicted levels of WS concentration, initial pH of the production medium, and yeast extract concentration provoking the ultimate xylanase levels (53.7 U/mL) with an 8.95-fold enhancement, localized by the estimated ridge of the steepest ascent of the ridge analysis path, were 3.8% (w/v), 5.1, and 0.098% (w/v), respectively; 94.7% lab validation. The current data underpin the up-scaling of xylanase production using this eco-friendly, cheap, and robust methodology for the valorization of WS into the value-added product xylanase.

Automated summary

This study highlights the enhanced production of high-value xylanase using a locally sourced novel Penicillium chrysogenum strain A3 DSM105774 through optimized statistical and sequential methods. Key factors prompting xylanase production were identified as incubation temperature, wheat straw, and ammonium sulfate, with WS concentration, yeast extract concentration, and initial pH of the production medium significantly affecting xylanase production. The predicted optimal conditions led to a significant increase in xylanase levels, showcasing the potential for upscaling xylanase production using an eco-friendly and cost-effective approach.