Journal
BIOACTIVE MATERIALS
Volume 10, Issue -, Pages 367-377Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2021.08.028
Keywords
Stimuli-responsive biomaterials; Shape memory polymer; Focal adhesion; Costamere; Dynamic mechanobiology; Human induced pluripotent stem cells
Funding
- NIH NICHD [R01HD101130]
- NSF [DMR-1609523, CMMI-2022421, CBET-1804875, CBET-1943798, CMMI-2130192]
- Syracuse University intramural CUSE Grant
- Gerber Grant
- BioInspired Institute Seed Grant
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This study utilized a shape memory polymer-based substrate to investigate the dynamic responsiveness of cell focal adhesions. By analyzing the dynamic changes of three proteins, the characteristics of cardiomyocyte focal adhesions were quantified, providing new insights into cardiac mechanobiology.
Focal adhesion complexes function as the mediators of cell-extracellular matrix interactions to sense and transmit the extracellular signals. Previous studies have demonstrated that cardiomyocyte focal adhesions can be modulated by surface topographic features. However, the response of focal adhesions to dynamic surface topographic changes remains underexplored. To study this dynamic responsiveness of focal adhesions, we uti-lized a shape memory polymer-based substrate that can produce a flat-to-wrinkle surface transition triggered by an increase of temperature. Using this dynamic culture system, we analyzed three proteins (paxillin, vinculin and zyxin) from different layers of the focal adhesion complex in response to dynamic extracellular topographic change. Hence, we quantified the dynamic profile of cardiomyocyte focal adhesion in a time-dependent manner, which provides new understanding of dynamic cardiac mechanobiology.
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