Journal
AQUACULTURE REPORTS
Volume 20, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aqrep.2021.100731
Keywords
Sex-linked SNP; Competitive allele-specific PCR assay; KASP; Portunus trituberculatus
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Funding
- National Key R&D Program of China [2018YFD0900303]
- Natural Science Foundation of Zhejiang Province [LY17C190005]
- Ministry of Agriculture of China & China Agriculture Research System [CARS-48]
- Scientific Research Foundation of Graduate School of Ningbo University [IF2020150]
- CSC Scholarship [201708330421]
- K.C. Wong Magana Fund in Ningbo University
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The study validated two sex-linked SNPs from simplified genomic data, providing a rapid test for sex identification in Portunus trituberculatus, and revealed significant genetic evidence from heterogametic genotypes in males supporting an XX/XY sex determination system.
The swimming crab Portunus trituberculatus is an economically vital aquaculture species in China and South East Asia. Monosex female culture has been identified as a key priority due to their increased market value, consumer preference and enhanced growth performance. However, the lack of a rapid and reliable sex-linked marker panel has hampered the application of sex control and understanding of the sex determination in the species. In this study, two sex-linked SNPs obtained from our simplified genomic data were validated by sequencing and then converted into competitive allele-specific PCR assays. The results showed high consistency between the genetic and phenotypic sex providing therefore a rapid test sex identification. Moreover, heterogametic genotypes in males provided a significant genetic evidence to support an XX/XY sex determination system in the P. trituberculatus.
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