4.6 Article

Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides

Journal

AQUACULTURE REPORTS
Volume 20, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.aqrep.2021.100734

Keywords

Lactobacillus casei; Micropterus salmoides; Growth performance; Gut health; Bacterial biota; Metabolomics

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Funding

  1. Science and Technology Commission of Shanghai Municipality [17050502200, 09320503600]
  2. National Natural Science Foundation of China [31671946]

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The study found that supplementing probiotics feed can significantly improve the growth performance, gut digestion and absorption, non-specific immune system, and gut bacterial community of Micropterus salmoides. Diets with Lactobacillus casei K17 as the main component can enhance the immune response of Micropterus salmoides, providing a reference for the feed industry.
Micropterus salmoides is one of the most economically valuable fish species in China. Currently, information in the effect of probiotics-supplemented feed on M. salmoides is rare. Present study investigated the impact of supplementary Lactobacillus casei K17 with six treatments on growth performance, gut digestion, absorption and non-specific immune, and gut bacterial biota and metabolomics of M. salmoides. A total of 450 M. salmoides (114 day of age, average 33.0 +/- 0.5 g) were randomly allocated into six groups for a 69-day rearing experiments. M. salmoides was given commercial feed supplemented with: 1) live L. casei K17 (1 & times; 109 CFU/g), 2) live L. casei K17 protected by skim milk powder (10 %), 3) heat-killed dead L. casei K17, 4) its fermentation supernatant, 5) skim milk powder (10 %), and 6) normal saline (0.85 % NaCl), designated as LB, MB, DB, FS, MG, and SG, respectively. MB and DB diets significantly increased body weight and growth rate, but decreased feed conversion ratio of M. salmoides (p < 0.05). These two diets also significantly increased gut amylase and lipase activities and microvilli surface areas (p < 0.05). Comparative bacterial biota analysis revealed distinct bacterial community compositions in M. salmoides gut contents among the six groups, which were remarkably dominated by L. casei in LB (90.0 %), MB (95.5 %), and DB (92.4 %) groups. Occurrences of some pathogenic bacteria were significantly reduced (p < 0.05). Meanwhile, up-modulated carbohydrate and down-modulated amino acid metabolisms were observed in LB, MB, and DB groups (p < 0.05). Comparative metabolomic analysis also revealed a number of notably altered metabolites, which, coupled with increased serum LZM and SOD, improved gut non-specific immune of M. salmoides, particularly in MB and DB groups. The results provided a reference of promising probiotic component L. casei K17 to feed industry.

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