Journal
ACS OMEGA
Volume 6, Issue 29, Pages 18745-18755Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.1c01795
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Funding
- National Natural Science Foundation of China [31701125]
- Shanxi Undergraduate Training Programs for Innovation and Entrepreneurship [2018595]
- Fund for Shanxi 1331 Project Key Subjects Construction [1331KSC]
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Saikosaponin D (SSD) can enhance cisplatin sensitivity of gastric cancer cells by inducing apoptosis and autophagy, and inhibiting the IKK beta/NF-κB signaling pathway.
Gastric cancer (GC) is the second leading cause of cancer deaths around the world. Chemoresistance is an important reason for poor prognosis of GC. Saikosaponin D (SSD) is a natural constituent from Radix Bupleuri and exhibits various activities including antitumors. This study investigated the effects and the mechanisms of SSD on cisplatin (cis-diamminedichloroplatinum, DDP) sensitivity of GC cells. Findings suggested that SSD could promote the inhibitory effect of DDP on proliferation and invasion and increase DDP-induced apoptosis in SGC-7901 and DDP-resistant cell line SGC-7901/DDP. We further identified that SSD increased levels of LC3 B and cleaved caspase 3 and decreased levels of p62, IKK beta, p-I kappa B alpha, and NF-kappa B p65, suggesting that SSD might inhibit the IKK beta/NF-kappa B pathway and induce both cell autophagy and apoptosis in SGC-7901 and SGC-7901/DDP. A further study indicated that SSD enhanced the effect of DDP-induced cleaved caspase 3 level rise and NF-kappa B pathway suppression, especially in SGC-7901/DDP cells. Conclusively, SSD enhanced DDP sensitivity of GC cells; the potential molecular mechanisms were that SSD-induced apoptosis and autophagy and inhibited the IKK beta/NF-kappa B pathway in GC cells. These findings suggested that SSD might contribute to overcoming DDP resistance in GC treatment.
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