Journal
PLANTS-BASEL
Volume 10, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/plants10081588
Keywords
barley; microspore; doubled haploid line; nitrogen use efficiency; RNA-seq
Categories
Funding
- Youth Talent Development Plan of Shanghai Municipal Agricultural System, China [20180133]
- National Natural Science Foundation of China [31801353]
- China Agriculture Research System of MOF [CARS-05-01A-02]
- SAAS Program for Excellent Research Team [2017 (B-01)]
- United Kingdom's Biotechnology and Biological Sciences Research Council (BBSRC) via the Designing Future Wheat Programme [BB/P016855/1]
- China Agriculture Research System of MARA [CARS-05-01A-02]
- BBSRC [BBS/E/C/000I0220] Funding Source: UKRI
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The study improved the nitrogen use efficiency (NUE) of barley line DH45 through F-1 microspore embryogenesis and hydroponic field experiments, and investigated the molecular mechanisms behind its superior NUE compared to its parents using RNA-seq analysis. The results revealed enrichment of genes involved in nitrogen compound metabolism in DH45 under low nitrogen conditions.
Creating varieties with high nitrogen use efficiency (NUE) is crucial for sustainable agriculture development. In this study, a superior barley doubled haploid line (named DH45) with improved NUE was produced via F-1 microspore embryogenesis with three rounds of screening in different nitrogen levels by hydroponic and field experiments. The molecular mechanisms responsible for the NUE of DH45 surpassing that of its parents were investigated by RNA-seq analysis. A total of 1027 differentially expressed genes (DEGs) were identified that were up- or down-regulated in DH45 under low nitrogen conditions but showed no significant differences in the parents. GO analysis indicated that genes involved in nitrogen compound metabolic processes were significantly enriched in DH45 compared with the parents. KEGG analysis showed the MAPK signaling pathway plant to be highly enriched in DH45 relative to its parents, as well as genes involved in alanine, aspartate and glutamate metabolism, and arginine biosynthesis. In conclusion, our study revealed the potential to fix trait superiority in a line by combining crossing with F-1 microspore culture technologies in future crop breeding and also identified several candidate genes that are expressed in shoots and may enable barley to cope with low-nitrogen stress.
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