The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber

Hydrogen gas (H-2) and glucose (Glc) have been reported as novel antioxidants and signal molecules involved in multiple biological processes in plants. However, the physiological roles and relationships of H-2 and Glc in adventitious rooting are less clear. Here, we showed that the effects of different concentrations Glc (0, 0.01, 0.05, 0.10, 0.50 and 1.00 mM) on adventitious rooting in cucumber were dose-dependent, with a maximal biological response at 0.10 mM. While, the positive roles of hydrogen rich water (HRW, a H-2 donor)-regulated adventitious rooting were blocked by a specific Glc inhibitor glucosamine (GlcN), suggesting that Glc might be responsible for H-2-regulated adventitious root development. HRW increased glucose, sucrose, starch and total sugar contents. Glucose-6-phosphate (G6P), fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P) contents were also increased by HRW. Meanwhile, the activities of sucrose-related enzymes incorporating sucrose synthase (SS) and sucrose phosphate synthase (SPS) and glucose-related enzymes including hexokinase (HK), pyruvate kinase (PK) and adenosine 5 '-diphosphate pyrophosphorylase (AGPase) were increased by HRW. Moreover, HRW upregulated the expression levels of sucrose or glucose metabolism-related genes including CsSuSy1, CsSuSy6, CsHK1, CsHK3, CsUDP1, CsUDP1-like, CsG6P1 and CsG6P1-like. However, these positive roles were all inhibited by GlcN. Together, H-2 might regulate adventitious rooting by promoting glucose metabolism.

Automated summary

Hydrogen gas (H-2) regulates adventitious rooting by promoting glucose metabolism in plants. Hydrogen rich water (HRW) increases glucose and sucrose content, enhances the activities of related enzymes, and upregulates the expression of genes involved in sucrose or glucose metabolism. These positive effects are all inhibited by a specific glucose inhibitor, suggesting a close relationship between H-2 and glucose in plant rooting processes.