Journal
PLANTS-BASEL
Volume 10, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/plants10081545
Keywords
Osmanthus fragrans var. aurantiacus; phillyrin; RAW 264.7 cells; antioxidant; optimal extraction; anti-inflammation; cytokine
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Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2020R1A2C1101252]
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This study aimed to identify the optimal extraction conditions for leaves of Osmanthus fragrans var. aurantiacus and compared the inhibitory effects of various extracts on NO production. The extracts showed antioxidant and anti-inflammatory properties, with Phillyrin (PH) identified as a major compound. OP OFLE reduced iNOS and COX-2 protein expression and downregulated proinflammatory cytokines, suggesting biological activities of the extracts and its major components.
The aim of this study was to identify the optimal extraction conditions for leaves of Osmanthus fragrans var. aurantiacus. Inhibitory effects of various extracts on NO production were compared. Antioxidant evaluations for total phenol and flavonoid contents were carried out using various extracts of O. fragrans var. aurantiacus leaves obtained under optimal extraction conditions that showed the greatest effect on NO production. The optimal method for extracting O. fragrans var. aurantiacus leaves resulted in an extract named OP OFLE. OP OFLE showed DPPH and ABTS radical scavenging activities in a concentration-dependent manner. Phillyrin (PH) was isolated as a major compound from OP OFLE by HPLC/DAD analysis. OP OFLE and PH reduced inducible nitric oxide (iNOS) and cyclooxygenase (COX)-2 protein expression and downregulated proinflammatory cytokines such as interleukin (IL)-1 beta, IL-6, IL-8, and tumor necrosis factor (TNF)-alpha in LPS-stimulated RAW 264.7 and HT-29 cells. To determine the signal pathway involved in the inhibition of NO production, a Western blot analysis was performed. Results showed that OP OFLE decreased phosphorylation of extracellular regulated kinase (pERK) 1/2 and the expression of nuclear factor-kappa B (NF-kappa B). Our results suggest that extracts of O. fragrans var. aurantiacus leaves and its major components have biological activities such as antioxidative and anti-inflammatory properties.
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