Journal
ANTIBIOTICS-BASEL
Volume 10, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/antibiotics10070770
Keywords
bacterial infection; Serratia marcescens; transcription factor; keratitis; ocular surface; epithelium; cornea; metabolomics
Categories
Funding
- Research to Prevent Blindness
- Eye and Ear Foundation of Pittsburgh
- National Institute of Health [P30EY08098, F32EY024785, T32EY017271, R01EY027331]
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This study investigates the impact of Serratia marcescens on corneal epithelial cell host responses, as well as the role of a bacterial transcription factor EepR in regulating host-pathogen interactions. Results show increased expression of proinflammatory and lipid signaling molecules in wild-type bacterial secretome-treated corneal cells.
Relatively little is known about how the corneal epithelium responds to vision-threatening bacteria from the Enterobacterales order. This study investigates the impact of Serratia marcescens on corneal epithelial cell host responses. We also investigate the role of a bacterial transcription factor EepR, which is a positive regulator of S. marcescens secretion of cytotoxic proteases and a hemolytic surfactant. We treated transcriptomic and metabolomic analysis of human corneal limbal epithelial cells with wild-type bacterial secretomes. Our results show increased expression of proinflammatory and lipid signaling molecules, while this is greatly altered in eepR mutant-treated corneal cells. Together, these data support the model that the S. marcescens transcription factor EepR is a key regulator of host-pathogen interactions, and is necessary to induce proinflammatory chemokines, cytokines, and lipids.
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