Journal
ANTIBIOTICS-BASEL
Volume 10, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/antibiotics10070854
Keywords
S; pseudintermedius; comparative genomics; host association; antimicrobial resistance
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This study compared the genetic relatedness and antimicrobial resistance genes of canine and human S. pseudintermedius isolates. The high genetic diversity in MSSP isolates from both hosts and the importance of multidrug resistance were highlighted, with CC241 being a prevalent multidrug resistance genotype. No host-specific genes were significantly associated with either human or canine isolates in this genomic analysis.
Staphylococcus pseudintermedius is an important pathogen in dogs that occasionally causes infections in humans as an opportunistic pathogen of elderly and immunocompromised people. This study compared the genomic relatedness and antimicrobial resistance genes using genome-wide association study (GWAS) to examine host association of canine and human S. pseudintermedius isolates. Canine (n = 25) and human (n = 32) methicillin-susceptible S. pseudintermedius (MSSP) isolates showed a high level of genetic diversity with an overrepresentation of clonal complex CC241 in human isolates. This clonal complex was associated with carriage of a plasmid containing a bacteriocin with cytotoxic properties, a CRISPR-cas domain and a pRE25-like mobile element containing five antimicrobial resistance genes. Multi-drug resistance (MDR) was predicted in 13 (41%) of human isolates and 14 (56%) of canine isolates. CC241 represented 54% of predicted MDR isolates from humans and 21% of predicted MDR canine isolates. While it had previously been suggested that certain host-specific genes were present the current GWAS analysis did not identify any genes that were significantly associated with human or canine isolates. In conclusion, this is the first genomic study showing that MSSP is genetically diverse in both hosts and that multidrug resistance is important in dog and human-associated S. pseudintermedius isolates.
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