Journal
FOODS
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/foods10061339
Keywords
dexamethasone; time-resolved fluorescent microspheres; immunochromatographic assay; milk; pork
Categories
Funding
- National Key Research and Development Program of China [2017YFC1601205]
- Natural Science Foundation of China [31701703, 31871883]
- Guangzhou Planned Program in Science and Technology [201803020026, 201807010109, 2017B020207010]
- Shanxi Provincial Key Research and Development Program [201803D221028-1]
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A time-resolved fluorescent microspheres immunochromatographic assay (TRFM-ICA) was developed for the quantitative detection of DEX in milk and pork, providing a new sensitive and efficient method for the rapid screening of DEX in food. The method showed high sensitivity and accuracy, with results comparable to LC-MS/MS analysis.
Dexamethasone (DEX) is widely used because of its anti-inflammatory, anti-endotoxin, anti-shock, and stress-enhancing response activities. It can increase the risk of diabetes and hypertension if it is abused or used improperly. However, there is a lack of sensitive and rapid screening methods for DEX in food. In this study, a time-resolved fluorescent microspheres immunochromatographic assay (TRFM-ICA) integrated with a portable fluorescence reader was developed for the quantitative detection of DEX in milk and pork. The cut-off values of the TRFM-ICA were 0.25 ng/mL and 0.7 mu g/kg, respectively. The limits of quantitation (LOQs) were 0.003 ng/mL and 0.062 mu g/kg, respectively. The recovery rates were 80.0-106.7%, and 78.6-83.6%, respectively, with the coefficients of variation ranging 6.3-12.5%, and 7.5-10.3%, respectively. A parallel experiment for 20 milk and 10 pork samples with LC-MS/MS was carried out to confirm the performance of the on-site application of the developed TRFM-ICA. The results of the two methods are basically the same; the correlation (R-2) was >0.98. The establishment of TRFM-ICA will provide a new sensitive and efficient technical support for the rapid screening of DEX in food.
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