Antioxidant and Anti-Inflammatory Effects of Zingiber officinale roscoe and Allium subhirsutum: In Silico, Biochemical and Histological Study

In this study, the antioxidant and anti-inflammatory effects of Zingiber officinale roscoe and Allium subhirsutum aqueous extracts were examined in a carrageenan-induced acute inflammation model. Some markers of inflammation such as hematological parameters, fibrinogen and C-reactive protein were measured. Variables reflecting oxidative stress included thiobarbituric acid reactive substances (TBARS), advanced oxidation of protein products (AOPP), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione were determined in both inflamed foci and erythrocytes. The in silico molecular docking simulation showed that the main components of Zingiber officinale roscoe and Allium subhirsutum bound to toll-like receptor 6 (TLR6) with high affinities. Moreover, histological examinations of paw edema were carried out. Both Zingiber officinale roscoe and Allium subhirsutum ameliorated the induced inflammation and oxidative stress status as outlined by anti-edematous, antioxidant and anti-inflammatory activities. Our investigation lends pharmacological support to the medical uses of these spices in the management of inflammatory disorders and oxidative damage. The results of the in silico assay satisfactory explain the in vivo effects as compared with indomethacin.

Automated summary

The study examined the antioxidant and anti-inflammatory effects of ginger and wild leek aqueous extracts in a carrageenan-induced acute inflammation model, along with measurements of inflammatory markers and oxidative stress variables. In silico molecular docking simulation revealed high affinities between the main components of ginger and wild leek with toll-like receptor 6 (TLR6). Histological examinations of paw edema showed that both extracts alleviated inflammation and oxidative stress, demonstrating anti-edematous, antioxidant, and anti-inflammatory activities. The results support the potential pharmacological use of these spices in managing inflammatory disorders and oxidative damage, with in silico assays effectively explaining the in vivo effects compared to indomethacin.