Journal
FOODS
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/foods10061192
Keywords
antimicrobial peptides; chickpea; chymotryptic hydrolysis; counter-ions; digestion optimization; Leg1; meat
Categories
Funding
- ADALBERT-RAPS-FOUNDATION
- GERMAN RESEARCH FOUNDATION (DFG) [INST 90/949-1 FUGG]
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The antimicrobial peptide Leg1 derived from chickpea legumin shows stable and effective activity against spoilage bacteria, yeast, and mold under food storage conditions. Its minimum inhibitory concentration (MIC) remains steady over time and is not affected by pH or temperature changes, suggesting potential for food preservation applications. Additionally, optimizing production methods could significantly increase the yield of Leg1, making it a promising food-grade agent.
The antimicrobial peptide Leg1 (RIKTVTSFDLPALRFLKL) from chickpea legumin is active against spoilage bacteria, yeast, and mold. The present study tested its effectiveness under food storage conditions and examined options to obtain a food-grade agent. The minimum inhibitory concentration (MIC) of Leg1 against E. coli (62.5 mu M) proved stable over seven days at 20 degrees C or 4 degrees C. It was not influenced by reduced pH (5.0 vs. 6.8), which is relevant in food such as meat. An incubation temperature of 20 degrees C vs. 37 degrees C reduced the MIC to 15.6/7.8 mu M against E. coli/B. subtilis. With a minimum bactericidal concentration in meat of 125/15.6 mu M against E. coli/B. subtilis, Leg1 is equivalently effective as nisin and 5000-82,000 times more active than sodium benzoate, potassium sorbate, or sodium nitrite. Replacing the counter-ion trifluoroacetate derived from peptide synthesis by the more natural alternatives acetate or chloride did not impair the activity of Leg1. As an alternative to chemical synthesis, an optimized protocol for chymotryptic hydrolysis was developed, increasing the yield from chickpea legumin by a factor of 30 compared to the standard procedure. The present results indicate that food-grade Leg1 could possibly be applicable for food preservation.
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