Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses

Although defensins have been isolated from a variety of metazoan, their role in cellular immunity has not been answered. In the study, we found that the hemocytes of the Manila clams Ruditapes philippinarum release defensin (designated as Rpdef3) in response to Vibrio parahaemolyticus challenge. The antimicrobial Rpdef3 was proved to be involved in the extracellular traps (ETs) that hemocytes released in response to Vibrio challenge. Scanning electron microscopy observation proved the patterns how ETs eliminate invading bacteria. Furthermore, Rpdef3 involved in ETs had broad-spectrum antimicrobial effect on both Gram-negative bacteria and Gram-positive bacteria. ELISA assay revealed that Rpdef3 could bind lipopolysaccharides and peptidoglycan in a dose-dependent manner. As concerned to the antibacterial mechanisms, Rpdef3 can cause bacterial membrane permeabilization, leading to cell death. As a result, Rpdef3 might contribute to the trap and the elimination of invading Vibrio in clam ETs. Taken together, our study suggest that the formation of ETs is a defense mechanism triggered by bacterial stimulation, coupled with antibacterial defensin.

Automated summary

The study found that hemocytes of Manila clams release the antimicrobial defensin Rpdef3 in response to Vibrio parahaemolyticus challenge, which is involved in the formation of extracellular traps to eliminate invading bacteria. Rpdef3 has broad-spectrum antimicrobial effects on both Gram-negative and Gram-positive bacteria by binding to lipopolysaccharides and peptidoglycan, leading to bacterial cell death through membrane permeabilization. This suggests that the formation of extracellular traps is a defense mechanism triggered by bacterial stimulation, coupled with the antibacterial defensin Rpdef3.