Journal
FRONTIERS IN MEDICINE
Volume 8, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmed.2021.689865
Keywords
lymphocyte subsets; hepatitis B virus; acute-on-chronic liver failure; immune response; flow cytometry
Categories
Funding
- National Natural Science Foundation of China [81770622, 81700559]
- Shaanxi Provincial Natural Science Foundation [2019JM-021]
- Clinical Research Award of the First AffiliatedHospital of Xi'an Jiaotong University, China [XJTU1AF-CRF-2018-002]
Ask authors/readers for more resources
This study found that peripheral lymphocyte count and percentage were significantly reduced in HBV-ACLF patients, as well as decreased CD8(+) T cell, CD4(+) T cell, and NK cell counts. A lower CD8(+) T cell count was associated with a higher mortality rate in HBV-ACLF patients, suggesting a potential prognostic indicator.
Background and Aims: Acute-on-chronic liver failure (ACLF) is a rare, but dramatic clinical syndrome. There is substantial evidence suggesting that immunity-mediated inflammation plays an important role in HBV-ACLF. Our aim was to characterize the proportion and cell counts of peripheral blood lymphocyte subsets in acute-on-chronic liver failure patients caused by HBV infection. Methods: One hundred and seventeen patients were enrolled in this study, including those with HBV-related ACLF (HBV-ACLF; n = 70), and HBV related non-ACLF patients (HBV non-ACLF; n = 47). Demographics, clinical and laboratory data at hospital admission were retrospectively analyzed. The percentage and cell count of peripheral lymphocyte subsets were evaluated by flow cytometry. Comparison analysis was performed by t-test or non-parametric Mann-Whitney U-test. Actuarial probabilities of death were calculated by the Kaplan-Meier method. Results: Both circulating lymphocyte count and lymphocyte percentage were significantly reduced in patients with HBV-ACLF (P < 0.001). The CD8(+) T cell, CD4(+) T cell, and CD16(+)CD56(+) NK cell counts were significantly decreased in HBV-ACLF. Consistently, flow cytometric analysis showed that CD8(+) T cell counts were significantly decreased in non-survivors, while no significant differences were found in CD4(+) T cell, CD19(+) B cell, or CD56(+)CD16(+) NK cell counts. Furthermore, the group with the lower CD8(+) T cell count displayed a significantly higher mortality rate compared with the group with the higher CD8(+) T cell count. Conclusions: The abnormal prevalence of lymphocyte subsets may be important in the pathogenesis of HBV-ACLF. The decrease in CD8(+) T cell counts may be related to poor survival in HBV-ACLF patients.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available