Journal
REGENERATIVE BIOMATERIALS
Volume 8, Issue 5, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/rb/rbab044
Keywords
gelatin methacrylate; bone marrow mesenchymal stem cells; solid lipid nanoparticles; resveratrol; bone regeneration
Categories
Funding
- Natural Science Foundation of Anhui Province [2008085QH362]
- Key Program of Anhui Educational Committee [KJ2020ZD51]
- Translational Medicine Key Projects of Bengbu Medical College [BYTM2019006, BYTM 2019012]
- Scientific Research Innovation Team of Bengbu Medical College [BYKC201910]
- 512 Talents Development Project of Bengbu Medical College [by51202302, by51202309]
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This study developed an innovative bone engineering scaffold based on GelMA hydrogel loaded with Res-SLNs, which demonstrated improved osteogenic differentiation of BMSCs, particularly with the optimal 0.02 Res-SLNs/GelMA scaffold. The slow and steady release of Res from the scaffold showed potential for application in bone tissue engineering.
Critical-sized bone defects caused by traumatic fractures, tumour resection and congenital malformation are unlikely to heal spontaneously. Bone tissue engineering is a promising strategy aimed at developing in vitro replacements for bone transplantation and overcoming the limitations of natural bone grafts. In this study, we developed an innovative bone engineering scaffold based on gelatin methacrylate (GelMA) hydrogel, obtained via a two-step procedure: first, solid lipid nanoparticles (SLNs) were loaded with resveratrol (Res), a drug that can promote osteogenic differentiation and bone formation; these particles were then encapsulated at different concentrations (0.01%, 0.02%, 0.04% and 0.08%) in GelMA to obtain the final Res-SLNs/GelMA scaffolds. The effects of these scaffolds on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and bone regeneration in rat cranial defects were evaluated using various characterization assays. Our in vitro and in vivo investigations demonstrated that the different Res-SLNs/GelMA scaffolds improved the osteogenic differentiation of BMSCs, with the ideally slow and steady release of Res; the optimal scaffold was 0.02 Res-SLNs/GelMA. Therefore, the 0.02 Res-SLNs/GelMA hydrogel is an appropriate release system for Res with good biocompatibility, osteoconduction and osteoinduction, thereby showing potential for application in bone tissue engineering.
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