Journal
PATHOGENS
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/pathogens10060662
Keywords
Mycobacterium; proteomics; cholesterol; infection; macrophages
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Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
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During tuberculosis infection, Mycobacterium utilizes host macrophage cholesterol for energy, and can induce cholesterol consumption in vitro by culturing in minimal medium. This leads to changes in cell wall compound accumulation, affecting pathogenicity. Proteomics analysis revealed that after cholesterol consumption, M. smegmatis can disrupt protein expression in macrophages, potentially impacting various cellular processes such as cytoskeleton remodeling, immune response, and immunometabolism.
During tuberculosis, Mycobacterium uses host macrophage cholesterol as a carbon and energy source. To mimic these conditions, Mycobacterium smegmatis can be cultured in minimal medium (MM) to induce cholesterol consumption in vitro. During cultivation, M. smegmatis consumes MM cholesterol and changes the accumulation of cell wall compounds, such as PIMs, LM, and LAM, which plays an important role in its pathogenicity. These changes lead to cell surface hydrophobicity modifications and H2O2 susceptibility. Furthermore, when M. smegmatis infects J774A.1 macrophages, it induces granuloma-like structure formation. The present study aims to assess macrophage molecular disturbances caused by M. smegmatis after cholesterol consumption, using proteomics analyses. Proteins that showed changes in expression levels were analyzed in silico using OmicsBox and String analysis to investigate the canonical pathways and functional networks involved in infection. Our results demonstrate that, after cholesterol consumption, M. smegmatis can induce deregulation of protein expression in macrophages. Many of these proteins are related to cytoskeleton remodeling, immune response, the ubiquitination pathway, mRNA processing, and immunometabolism. The identification of these proteins sheds light on the biochemical pathways involved in the mechanisms of action of mycobacteria infection, and may suggest novel protein targets for the development of new and improved treatments.
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