4.6 Review

Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis

Journal

FRONTIERS IN MOLECULAR BIOSCIENCES
Volume 8, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2021.682405

Keywords

COVID-19 diagnosis; serological testing; antibody; SARS-CoV-2; ELISA; LFIA

Funding

  1. National Natural Science Foundation of China [81974258]
  2. COVID-19 special task grant from Chinese Academy of Sciences Clinical Research Hospital (Hefei) [YD9110002006]
  3. Fundamental Research Funds for the Central Universities [WK9110000092]

Ask authors/readers for more resources

The global COVID-19 pandemic has led to the development of various clinical diagnosis methods, including nucleic acid testing and serological screening. Due to the unsatisfactory sensitivity of nucleic acid detection, serological screening has become widely used. These serological diagnostic methods help improve clinical efficiency and control the spread of COVID-19.
The worldwide pandemic of COVID-19 has become a global public health crisis. Various clinical diagnosis methods have been developed to distinguish COVID-19-infected patients from healthy people. The nucleic acid test is the golden standard for virus detection as it is suitable for early diagnosis. However, due to the low amount of viral nucleic acid in the respiratory tract, the sensitivity of nucleic acid detection is unsatisfactory. As a result, serological screening began to be widely used with the merits of simple procedures, lower cost, and shorter detection time. Serological tests currently include the enzyme-linked immunosorbent assay (ELISA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA). This review describes various serological methods, discusses the performance and diagnostic effects of different methods, and points out the problems and the direction of optimization, to improve the efficiency of clinical diagnosis. These increasingly sophisticated and diverse serological diagnostic technologies will help human beings to control the spread of COVID-19.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available