Journal
FRONTIERS IN MOLECULAR BIOSCIENCES
Volume 8, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2021.712971
Keywords
DNA replication; replisome; DNA damage tolerance; translesion synthesis; repriming; recoupling; replication fork
Categories
Funding
- Wellcome Trust [213596/Z/18/Z]
- Medical Research Council as part of the United Kingdom Research and Innovation [MC_UP_1201/12]
- Wellcome Trust [213596/Z/18/Z] Funding Source: Wellcome Trust
Ask authors/readers for more resources
The eukaryotic replisome encounters obstacles such as damaged and structured DNA during replication, but various mechanisms exist to overcome these hurdles, maintain progression, and prevent fork collapse. Recent advances in structural, biochemical, and single-molecule approaches have enhanced our understanding of the replisome's architecture, response to impediments, and DNA damage tolerance mechanisms.
The eukaryotic replisome coordinates template unwinding and nascent-strand synthesis to drive DNA replication fork progression and complete efficient genome duplication. During its advancement along the parental template, each replisome may encounter an array of obstacles including damaged and structured DNA that impede its progression and threaten genome stability. A number of mechanisms exist to permit replisomes to overcome such obstacles, maintain their progression, and prevent fork collapse. A combination of recent advances in structural, biochemical, and single-molecule approaches have illuminated the architecture of the replisome during unperturbed replication, rationalised the impact of impediments to fork progression, and enhanced our understanding of DNA damage tolerance mechanisms and their regulation. This review focusses on these studies to provide an updated overview of the mechanisms that support replisomes to maintain their progression on an imperfect template.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available