4.6 Article

In Vitro Anti-Biofilm Activity of Bacteriophage K (ATCC 19685-B1) and Daptomycin against Staphylococci

Journal

MICROORGANISMS
Volume 9, Issue 9, Pages -

Publisher

MDPI
DOI: 10.3390/microorganisms9091853

Keywords

staphylococci; biofilm; prevention; phage K; daptomycin

Categories

Funding

  1. Research Committee of the University of Patras, Greece [39540000]
  2. Institute of Chemical Engineering Sciences, FORTH/ICE-HT: the Operational Program Human Resources Development, Education and Lifelong Learning 2014-2020
  3. European Union (European Social Fund-ESF) [39540000, MIS 5049223]
  4. Operational Program Competitiveness, Entrepreneurship and Innovation (NSRF 2014-2020)
  5. European Union (European Regional Development Fund) [MIS 5031792]

Ask authors/readers for more resources

The study investigated the anti-staphylococcal activity of daptomycin and bacteriophage K, alone or in combination, against S. aureus and S. epidermidis strains. Best results were achieved when both agents were combined in high concentrations, with bacteriophage K showing effectiveness within a few hours depending on concentration. The combination was more effective against staphylococci, suggesting it may be a potential choice for biofilm-associated infections prevention or therapy.
The purpose of the present study was to investigate anti-staphylococcal activity of daptomycin and bacteriophage K, alone or in combination, against biofilm-producers and non-producers S. aureus and S. epidermidis strains, under biofilm forming and cells' proliferation conditions. Daptomycin and bacteriophage K (ATCC 19685B1), in different concentrations, were tested against 10 Staphylococcus aureus and 10 S. epidermidis, characterized by phenotypes and genotypes. The quantitative microtiter plate (crystal violet, CV), methylthiazoltetrazolium (MTT), and growth curve (GC) assays were performed. No statistically significant difference was found between species, whereas daptomycin alone performed better using medium and high concentrations of the drug and bacteriophage K was more active against strains with higher susceptibility, by CV and MTT assays. Best results were achieved using both agents combined in high concentrations. Bacteriophage K was effective within 3.8 and 2.4 h, depending on the concentration used, by the GC assay. Combination of daptomycin with bacteriophage K was more effective against staphylococci, depending on the concentrations used and strains' susceptibility. Further studies are needed to evaluate if this approach might be a choice for prevention or therapy of biofilm-associated infections.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available