Journal
MICROORGANISMS
Volume 9, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms9071403
Keywords
Taenia pisiformis cysticercus; exosome; miRNA let-7-5p; macrophages polarization
Categories
Funding
- National Key Research and Development Program of China [2017YFD0501303]
- National Natural Science Foundation of China [32072889, 31772726]
- Central Public-interest Scientific Institution Basal Research Fund [1610312020017]
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The study reveals that miR-let-7-5p released by Taenia pisiformis cysticercus plays a critical role in activating M2 macrophages, inducing M2 macrophage polarization by targeting C/EBP delta.
Cysticercus pisiformis, the larval stage of Taenia pisiformis, causes serious illness in rabbits that severely impacts the rabbit breeding industry. An inhibitive Th2 immune response can be induced by let-7-enriched exosomes derived from T. pisiformis cysticercus. However, the underlying molecular mechanisms are not completely understood. Here, we report that exosomal miR-let-7-5p released by T. pisiformis cysticercus played a critical role in the activation of M2 macrophages. We found that overexpression of let-7-5p in M1 macrophages decreased M1 phenotype expression while promoting polarization to the M2 phenotype, which is consistent with experimental data in exosome-treated macrophages alone. In contrast, knockdown of let-7-5p in exosome-like vesicles promoted M1 polarization and decreased M2 phenotype expression. Furthermore, down-regulation of transcription factor CCAAT/enhancer-binding protein (C/EBP)-delta resulted in the decrease of M1 phenotype markers and increase of M2 phenotype markers. These results suggested that let-7 enriched in exosome-like vesicles from T. pisiformis metacestodes can induce M2 macrophage polarization via targeting C/EBP delta, which may be involved in macrophage polarization induced by T. pisiformis metacestodes. The finding helps to expand our knowledge of the molecular mechanism of immunosuppression and Th2 immune response induced by metacestodes.
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