Journal
MICROORGANISMS
Volume 9, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms9061228
Keywords
clinical strains; miltefosine; susceptibility; visceral leishmaniasis
Categories
Funding
- Drugs for Neglected Diseases initiative
- Associacao Bem-Te-Vi Diversidade, Brazil
- UK aid, UK
- Dutch Ministry of Foreign Affairs (DGIS), the Netherlands
- Medecins sans Frontieres
- Swiss Agency for Development and Cooperation (SDC), Switzerland
- CNPq - National Council for Scientific and Technological Development [302622/2017-9, 306971/2018-6]
- FAPERJ-Carlos Chagas Filho Research Foundation of Rio de Janeiro State [E26/202.569/2019]
- PAEF-IOC-FIOCRUZ
- Oswaldo Cruz Foundation
- FAPESP-Fundacao de Apoio a Pesquisa do Estado de Sao Paulo [2015/090802, 2016/23405-4]
- Capes-Brazilian Federal Agency for Support and Evaluation of Graduate Education [001]
- CNPq
Ask authors/readers for more resources
This study investigated the susceptibility of 73 Brazilian strains of Leishmania infantum to miltefosine, showing relatively low heterogeneity in miltefosine susceptibility. The results suggest that Brazilian L. infantum may not have decreased susceptibility to miltefosine, indicating the potential for future clinical evaluation of miltefosine for VL treatment in Brazil.
Treatment of visceral leishmaniasis in Brazil still relies on meglumine antimoniate, with less than ideal efficacy and safety, making new therapeutic tools an urgent need. The oral drug miltefosine was assayed in a phase II clinical trial in Brazil with cure rates lower than previously demonstrated in India. The present study investigated the susceptibility to miltefosine in 73 Brazilian strains of Leishmania infantum from different geographic regions, using intracellular amastigote and promastigote assays. The EC50 for miltefosine of 13 of these strains evaluated in intracellular amastigotes varied between 1.41 and 4.57 mu M. The EC50 of the 73 strains determined in promastigotes varied between 5.89 and 23.7 mu M. No correlation between in vitro miltefosine susceptibility and the presence of the miltefosine sensitive locus was detected among the tested strains. The relatively low heterogeneity in miltefosine susceptibility observed for the 73 strains tested in this study suggests the absence of decreased susceptibility to miltefosine in Brazilian L. infantum and does not exclude future clinical evaluation of miltefosine for VL treatment in Brazil.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available