Journal
ANTIOXIDANTS
Volume 10, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/antiox10091345
Keywords
Carissa carandas; ursolic acid; antioxidant; anti-tyrosinase; matrix metalloproteinases; nuclear factor-kappa B; interleukin; human epidermal keratinocyte line
Funding
- Graduate School, Chiang Mai University, Thailand
- Faculty of Pharmacy, Chiang Mai University, Thailand
- Research Center of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Chiang Mai University
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This study revealed the potential of Carissa carandas as a natural anti-aging, antioxidant, and skin whitening agent. The leaf extract had the highest content of ursolic acid and showed the most significant inhibitory effects on MMP-1, NF-kappa B, and tyrosinase activity. Ursolic acid was suggested to be a key component in these biological activities, with the potential for use in cosmetics and cosmeceuticals.
In this study, the potential of Carissa carandas Linn. as a natural anti-aging, antioxidant, and skin whitening agent was studied. Various parts of C. carandas, including fruit, leaf, seed, and pulp were sequentially extracted by maceration using n-hexane, ethyl acetate, and ethanol, respectively. High-performance liquid chromatography, Folin-Ciocalteu, and Dowd method were used to investigate their chemical compositions. The inhibitory activities of oxidation process, matrix metalloproteinases (MMPs), elastase, hyaluronidase, and tyrosinase were analyzed. Cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay in a human epidermal keratinocyte line (HaCaT). The results exhibited that ethyl acetate could extract the most ursolic acid from C. carandas, while ethanol could extract the most phenolics and flavonoids. The leaf extract had the highest content of ursolic acid, phenolics, and flavonoids. The leaf extracted with ethyl acetate (AL) had the highest ursolic acid content (411.8 mg/g extract) and inhibited MMP-1, NF-kappa B, and tyrosinase activity the most. Ursolic acid has been proposed as a key component in these biological activities. Although several C. carandas extracts are beneficial to human skin, AL has been proposed for use in cosmetics and cosmeceuticals due to its superior anti-wrinkle, anti-inflammation, and whitening properties.
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