Journal
ANTIOXIDANTS
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/antiox10060930
Keywords
lifespan; anthocyanins; phytochemicals; HSP-1 pathway; CaMKII pathway; HSP-1 pathway; caco-2 cells
Funding
- Key-Area Research and Development Program of Guangdong Province [2018B020239001]
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The study demonstrated that the derivative of CY3D5G from red cabbage juice has protective effects against oxidative stress and improves cell viability, as well as extends the lifespan of Caenorhabditis elegans. These effects may be attributed to the higher content of antioxidants in red cabbage juice compared to green cabbage juice.
Many studies have demonstrated that cabbages possess various biological activities, and our previous studies confirmed that cyanidin-3-diglucoside-5-glucoside (CY3D5G), the major core of red cabbage anthocyanins, exhibited in vitro antioxidant activity. This study further investigated the protective effects of CY3D5G derivative from red cabbage juice (RCJ) on oxidative stress and lifespan in cells and Caenorhabditis elegans, green cabbage juice (GCJ) was used as control. RCJ rather than GCJ significantly improved cell viability and decreased lactate dehydrogenase release in H2O2-induced caco-2 cells. RCJ significantly increased survival during oxidative and heat stress and mean lifespan in C. elegans by 171.63% and 31.64%, and 28.16%, respectively, while GCJ treatment showed no significant effects (p < 0.05). These results might be attributed to significantly (p < 0.05) higher contents of total phenolics, ascorbic acid, glucosinolates, and anthocyanins in RCJ compared to those in GCJ. Additionally, both of them decreased autofluorescence and reproductive capacity, increased body length, but did not alter the intracellular ROS level. Prolonged lifespan by RCJ might require heat-shock transcription factor pathway, sirtuin signaling, and calmodulin kinase II pathway, independent of insulin/insulin-like growth factor-1 signaling pathway. RCJ showed promising antioxidant properties in caco-2 cells and C. elegans, which provided more information on the health benefits of cabbage.
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