Journal
BIOMOLECULES
Volume 11, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/biom11091306
Keywords
mycobacterium tuberculosis; dendritic cell; Rv1876; Th1 polarization; BCG-prime boost
Categories
Funding
- National Research Foundation of Korea (NRF) - Ministry of Education [2016R1A6A3A11934237, 2019R1I1A1A01040723]
- Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic Korea [HI17C0175]
- National Research Foundation of Korea [2019R1I1A1A01040723, 2016R1A6A3A11934237] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Studies have shown that the Rv1876 protein identified from Mtb culture filtrates can enhance DCs and Th1 cell immune responses, reducing bacterial load in the lungs when used in conjunction with the BCG vaccine.
The widely administered tuberculosis (TB) vaccine, Bacillus Calmette-Guerin (BCG), is the only licensed vaccine, but has highly variable efficiency against childhood and pulmonary TB. Therefore, the BCG prime-boost strategy is a rational solution for the development of new TB vaccines. Studies have shown that Mycobacterium tuberculosis (Mtb) culture filtrates contain proteins that have promising vaccine potential. In this study, Rv1876 bacterioferritin was identified from the culture filtrate fraction with strong immunoreactivity. Its immunobiological potential has not been reported previously. We found that recombinant Rv1876 protein induced dendritic cells' (DCs) maturation by MAPK and NF-kappa B signaling activation, induced a T helper type 1 cell-immune response, and expanded the population of the effector/memory T cell. Boosting BCG with Rv1876 protein enhanced the BCG-primed Th1 immune response and reduced the bacterial load in the lung compared to those of BCG alone. Thus, Rv1876 is a good target for the prime-boost strategy.
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