Journal
BIOMOLECULES
Volume 11, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/biom11081075
Keywords
cyanine dye; DNA binding; RNA binding; fluorescence; circular dichroism; antiproliferative activity
Categories
Funding
- Croatian Science Foundation [IP-2018-01-5475]
- Bulgarian National Science Fund (BNSF) project SOFIa [KP-06-H39/11]
Ask authors/readers for more resources
The new analogs of commercial cyanine dyes exhibited strong affinities to various nucleic acids, especially towards GC-DNA base pairs. They showed intercalation properties at low dye-DNA/RNA ratios and potent antiproliferative activity against cancer cell lines, making them promising theranostic agents.
New analogs of the commercial asymmetric monomethine cyanine dyes thiazole orange (TO) and thiazole orange homodimer (TOTO) with hydroxypropyl functionality were synthesized and their properties in the presence of different nucleic acids were studied. The novel compounds showed strong, micromolar and submicromolar affinities to all examined DNA ds-polynucleotides and poly rA-poly rU. The compounds studied showed selectivity towards GC-DNA base pairs over AT-DNA, which included both binding affinity and a strong fluorescence response. CD titrations showed aggregation along the polynucleotide with well-defined supramolecular chirality. The single dipyridinium-bridged dimer showed intercalation at low dye-DNA/RNA ratios. All new cyanine dyes showed potent micromolar antiproliferative activity against cancer cell lines, making them promising theranostic agents.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available