Preparation of Affinity Purified Antibodies against ε-Glutaryl-Lysine Residues in Proteins for Investigation of Glutarylated Proteins in Animal Tissues

The glutarylation of lysine residues in proteins attracts attention as a possible mechanism of metabolic regulation, perturbed in pathologies. The visualization of protein glutarylation by antibodies specific to epsilon-glutaryl-lysine residues may be particularly useful to reveal pathogenic mutations in the relevant enzymes. We purified such antibodies from the rabbit antiserum, obtained after sequential immunization with two artificially glutarylated proteins, using affinity chromatography on epsilon-glutaryl-lysine-containing sorbents. Employing these anti(epsilon-glutaryl-lysine)-antibodies for the immunoblotting analysis of rat tissues and mitochondria has demonstrated the sample-specific patterns of protein glutarylation. The study of the protein glutarylation in rat tissue homogenates revealed a time-dependent fragmentation of glutarylated proteins in these preparations. The process may complicate the investigation of potential changes in the acylation level of specific protein bands when studying time-dependent effects of the acylation regulators. In the rat brain, the protein glutarylation, succinylation and acetylation patterns obtained upon the immunoblotting of the same sample with the corresponding antibodies are shown to differ. Specific combinations of molecular masses of major protein bands in the different acylation patterns confirm the selectivity of the anti(epsilon-glutaryllysine)-antibodies obtained in this work. Hence, our affinity-purified anti(epsilon-glutaryllysine)-antibodies provide an effective tool to characterize protein glutarylation, revealing its specific pattern, compared to acetylation and succinylation, in complex protein mixtures.

Automated summary

The study focuses on the glutarylation of lysine residues in proteins, revealing sample-specific patterns of protein glutarylation in rat tissues and mitochondria. Time-dependent fragmentation of glutarylated proteins in rat tissue homogenates was observed, complicating the investigation of acylation regulators' effects. The affinity-purified anti(epsilon-glutaryllysine)-antibodies proved to be an effective tool for characterizing protein glutarylation and showing its specific pattern compared to other acylation types in complex protein mixtures.